Pennycress (Thlaspi arvense) a winter annual weed, is undergoing domestication as a winter cover and oilseed crop for corn-soybean rotation in the upper Midwest. While previous studies have primarily delved into the agricultural aspects of pennycress cultivation, the potential impact of insect pests on this crop has been overlooked. Nevertheless, there are documented cases of various insect herbivores targeting pennycress when it grows as a weed. Among these, the diamondback moth (Plutella xylostella) is a notorious pest of brassica crops, posing a significant threat to plants such as cabbage, broccoli, and cauliflower. To assess the feeding preference and survival of the diamondback moth on pennycress, laboratory experiments were conducted as choice and no-choice tests. Two pennycress genotypes, MN106 (wild type) and AOP (low-glucosinolate mutant), were used in these experiments. The results of these experiments are currently being analyzed. The overarching goal of the project is to evaluate the potential risks posed by insect pests to pennycress, with the aim of contributing to the development of effective management strategies for this emerging crop.