Biscayne National Park (BISC) and the Florida Fish and Wildlife Conservation Commission (FWC) began collaboratively implementing the Biscayne Fishery Management Plan (FMP) in 2020. Included among a variety of regulatory changes that went into effect to support the plan was the creation of a No-Trawl Zone (NTZ) within Biscayne Bay. Upon completion of the FMP, BISC and FWC developed the FMP Science Plan, which is the guiding document to assess the efficacy of the implemented regulations; the FMP Assessment Report is scheduled for completion in 2027. This proposed study will provide critical data for the FMP Assessment Report, by examining changes to benthic habitats with respect to the implementation of the NTZ, trawling fishing effort and landings, and comparing habitat health and fish communities in the Biscayne Bay NTZ and adjacent trawled areas to determine if the NTZ is effectively protecting habitat and wildlife, or if additional regulatory measures (i.e., expansion or modification) are needed.