Over the years, sport fishing has shown significant growth, but this activity can have negative effects on fish returned to their natural environment, resulting in immediate or delayed mortality. Thus, the objective of this study is to understand the perception of sport fishers regarding the practices adopted during the capture and release of specimens. Interviews were conducted using semi-structured questionnaires, individually, through conversations with fishers operating in the Rondônia region (n=18). Among the respondents, 72.2% are from Porto Velho, 11.1% from Pimenta Bueno, and 11.1% from Guajará-Mirim. Most of the fishers are men, representing 88.9% of the total. In terms of age, 33.3% are between 40 and 50 years old. Regarding education, 44.4% have completed higher education, while 22.2% have finished high school. In terms of income, 39.9% earn more than 10 times the minimum wage. Regarding fishing practices, 61.1% of respondents believe that fish are most frequently injured during capture and boarding, and 72.2% claim to have knowledge of best fishing practices. The Jamari and Jaci rivers were the most mentioned as preferred fishing locations, each with 21.6% of the responses. Finally, 30.8% of fishers pointed out that the use of barbless hooks causes less harm to fish, with Tucunaré (10.5%) and Jatuarana (9.3%) being the most commonly caught species. The data suggest that, despite the good level of knowledge about best practices among sport fishers, there is still room for improvement in the management of captured species, minimizing the potential harm caused by the activity.